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Image Search Results
Journal: Journal of medicinal chemistry
Article Title: Aryl Trehalose Derivatives as Vaccine Adjuvants for Mycobacterium tuberculosis
doi: 10.1021/acs.jmedchem.9b01598
Figure Lengend Snippet: Induction of IL-17 producing CD4+ T cells from UM1024 adjuvanted M72 vaccine. BALB/c mice, 10 per group, were immunized two times i.m. with 0.1 μg antigen plus 2, 10 or 50 nmol of the indicated adjuvant. Spleens were harvested from 3 mice per group at 5 days post-secondary vaccination. Splenocytes were restimulated with 1 μg whole antigen and transport inhibitors followed by surface staining for CD3, CD4 and CD8 and intracellular cytokine staining for IL-17A. Data represent percentage of live, CD3+/CD4+ cells that are also positive for IL-17A upon antigen restimulation.
Article Snippet: Following incubation, cells were stained with the cell surface antibodies against CD3e PerCP-Cy5.5 (Tonbo Biosciences, 145–2C11), CD4a APC-Cy7 (Tonbo Biosciences, RM4–5) and
Techniques: Adjuvant, Staining